EGFR Antibody

SKU: orb1253732

Description

EGFR Antibody

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: EGFR (Phospho-Tyr1197) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y (p) -L-R) derived from Human EGFR.
• Target: EGFR
• Molecular Weight: 175 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERBB, HER1, mENA, ERBB1, PIG61, MADH2, MADR2, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR

Western blot analysis of lysed extracts from HUVEC cells untreated or treated with EGF using EGFR (Phospho-Tyr1197).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1197).

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

RefSeq (Protein):NP_005219.2