Amivantamab Biosimilar Antibody, Research Grade (EGFR/c-Met)

SKU: orb2977888

Description

Amivantamab is a research-grade recombinant antibody targeting HER1. Produced in mammalian cells, it features native-like glycosylation and is suitable for PK/PD studies, assay development, and ADA research.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Antibody Type: Biosimilar Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1-kappa
• Target: HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB, Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET
• Source/Expression System: Mammalian Cells
• Purity: >95% purity as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE for Research Grade Amivantamab.

SEC-HPLC detection for Research Grade Amivantamab.

Detects EGFR/ERBB1/HER1 in indirect ELISAs.

Detects recombinant human Met (Cat No.: orb2965006) in indirect ELISA.

Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: orb2977888, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: orb2977888, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: orb2977888, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog: orb2977888) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

Gene Symbol

HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB, Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET