EGFR Mouse mAb

• Catalog Number: orb763473
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1mg/mL
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
• Immunogen: Synthetic Peptide
• UniProt ID: P00533
• MW: 170kDa
• Tested applications: IF, IHC, IP, WB
• Dilution range: WB 1:1,000-3,000 IP 1:200 IF 1:100-200
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Epidermal Growth Factor Receptor antibody, an
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 1) A431 , 2) Hela, with orb763473 diluted at 1:2, 000.

1: Input: Hela Cell Lysate 2: IP product: IP dilute 1:200 Western blot analysis: primary antibody : orb763473 1:1, 000 Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific (S003), 1:5, 000.

IF analysis of Hela with orb763473 (Left) and DAPI (Right) diluted at 1:100.