EGFR Antibody (C-term)

SKU: orb1788111

Description

Mouse Monoclonal Antibody (Mab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Immunogen: This EGFR antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1163-1191 amino acids from the C-terminal region of human EGFR. Antigen Region: 1163-1191 aa.
• Target: EGFR (HGNC:3236)
• Molecular Weight: 134277 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

All lanes: Anti-EGFR Antibody (C-term) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

EGFR (HGNC:3236)

UniProt

P00533

RefSeq (Protein):NP_958441.1, NP_958439.1, NP_005219.2, NP_958440.1