EGFR Antibody

SKU: orb345526

Description

EGFR antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8,000 - 1:32,000, WB: 1:500 - 1:5,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to EGFR protein by western blotting in the appropriate cell lysate or extract. This antibody detects both non-phosphorylated and phosphorylated EGFR at residue Y1197.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus near amino acids 1175-1200 of human EGFR protein.
• Target: EGFR
• Purity: EGFR Antibody is directed against human epidermal growth factor receptor (EGFR) and is useful in determining its presence in ELISA and western blotting experiments. This antibody can detect EGFR from human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-EGFR at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.99 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-EGFR antibody, Epidermal growth factor receptor antibody, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erbb 1, Erbb-1, Erbb1, HER1 antibody

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

RefSeq:29725609