EGFR Antibody

SKU: orb345526

Description

EGFR antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8,000 - 1:32,000, WB: 1:500 - 1:5,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to EGFR protein by western blotting in the appropriate cell lysate or extract. This antibody detects both non-phosphorylated and phosphorylated EGFR at residue Y1197.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus near amino acids 1175-1200 of human EGFR protein.
• Purity: EGFR Antibody is directed against human epidermal growth factor receptor (EGFR) and is useful in determining its presence in ELISA and western blotting experiments. This antibody can detect EGFR from human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-EGFR at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.99 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-EGFR antibody, Epidermal growth factor receptor antibody, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erbb 1, Erbb-1, Erbb1, HER1 antibody

Western Blot of Rabbit anti-EGFR antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348668). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lysate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 35 µg per lane. Primary antibody: EGFR antibody at 1 ug/ml overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 170kDa for EGFR.

Western blot using Biorbyt's Affinity Purified anti-EGFR antibody shows detection of a band at ~170 kDa corresponding to human EGFR (arrowhead). Lane 1: unstimulated A431 whole cell lysate (p/n orb348665). Lane 2: EGF (50 ng/mL for 15 min) stimulated A431 whole cell lysates (p/n orb348666). Approximately 30 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4°C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).

Quick Database Links

UniProt

P00533

RefSeq:29725609