EGFR Antibody

SKU: orb1537913

Description

EGFR Antibody

Images & Validation

• Tested Applications: ICC, IHC, IHC-P, IP, WB
• Dilution Range: ICC, IHC, IHC-P (5 µg/ml), IP (10 - 12 µg/ml), WB
• Reactivity: Gallus, Human, Mouse, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration.

Key Properties

• Host: Sheep
• Clonality: Polyclonal
• Immunogen: A synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region
• Target: EGFR
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Quick Database Links

Gene Symbol

EGFR