EGFR Antibody

• Catalog Number: orb1537913
• Category: Antibodies
• Description: EGFR Antibody
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Sheep
• Conjugation: Unconjugated
• Reactivity: Gallus, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS. PBS
• Purification: Protein G purified
• Immunogen: A synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region
• Tested applications: ICC, IHC, IHC-P, IP, WB
• Dilution range: ICC, IHC, IHC-P (5 µg/ml), IP (10 - 12 µg/ml), WB
• Application notes: Further information: Immunohistochemistry: LS-B2914 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2914 was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.