EGFR Antibody

• Catalog Number: orb153504
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Immunogen: Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.
• UniProt ID: P00533
• MW: 175kDa
• Tested applications: FC, WB
• Dilution range: WB: 1/500 - 1/2000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Antibody Type: Primary Antibody
• Clone Number: 5E10D3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; HER1; mENA; ERBB1; PIG61
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR antibody

Flow cytometric analysis of A431 cells lysates using EGFR antibody

Western blot analysis of human EGFR using EGFR antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR antibody

Western blot analysis of A431 (Lane 1) Hela (Lane 2) cell lysate using EGFR antibody