EGFR Antibody

• Catalog Number: orb153504
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5E10D3
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.
• Concentration: 1mg/ml
• Dilution range: WB: 1/500 - 1/2000; RCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 175kDa
• Entrez: 1956
• Storage: 4°C; -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: ERBB; HER1; mENA; ERBB1; PIG61
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR antibody

Flow cytometric analysis of A431 cells lysates using EGFR antibody

Western blot analysis of human EGFR using EGFR antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR antibody

Western blot analysis of A431 (Lane 1) Hela (Lane 2) cell lysate using EGFR antibody