EGFR Antibody

SKU: orb1534833

Description

EGFR Antibody

Images & Validation

• Tested Applications: IHC, IHC-P
• Dilution Range: IHC, IHC-P (2.5 - 5 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic 16 amino acid peptide from C-Terminus of human EGFR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Marmoset, Rat (94%); Mouse (88%); Bovine, Dog, Hamster, Elephant, Horse (81%).
• Target: EGFR
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide
• Concentration: 0.8 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Human Breast Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

Quick Database Links

Gene Symbol

EGFR