EGFR Antibody

• Catalog Number: orb1263070
• Category: Antibodies
• Description: EGFR Antibody
• Target: EGFR
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from human EGFR.
• UniProt ID: P00533
• MW: 134 kDa
• Tested applications: IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Epidermal growth factor receptor, Proto-oncogene c
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only
• NCBI: P00533
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-EGFR Antibody in kidney heart tissue lysates (35 ug/lane)

Western blot analysis of EGFR in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EGFR Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.