EGFR (Ab-1172) Antibody

• Catalog Number: orb685387
• Category: Antibodies
• Description: This EGFR (Ab-1172) Antibody is an unconjugated polyclonal product. It tagets EGFR using a Synthesized non-phosphopeptide derived from Human EGFR around the phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q) as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB. Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen..
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: liquid
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized non-phosphopeptide derived from Human EGFR around the phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q).
• UniProt ID: P00533
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: WB: 1: 500-1: 3000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-EC 2.7.10.1 antibody, anti-epidermal growth f
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A549 cells (Lane 2) and HepG2 cells (Lane 3) using EGFR (Ab-1172) antibody

Immunohistochemical staining of human breast carcinoma tissue using EGFR (Ab-1172) antibody

Western blot analysis of HT-29 cells using EGFR (Ab-1172) antibody