EGFR (5E10) Mouse mAb

SKU: orb1563166

Description

EGFR (5E10) Mouse mAb

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IP, WB
• Dilution Range: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5E10-G10-E11
• Immunogen: Purified recombinant human EGFR protein fragments expressed in E.coli.
• Target: EGFR
• Molecular Weight: Calculated MW: 134 kDa; Observed MW: 175 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Western blot analysis of EGFR in A549, MDA-MB-468 and COS7 lysates using EGFR antibody.

Immunocytochemistry analysis of EGFR (5E10) in HeLa using EGFR antibody.

Immunoprecipitation analysis of EGFR (5E10) in Hela lysates using EGFR antibody.

Immunocytochemistry analysis of EGFR in MDA-MB-468 cells using EGFR antibody.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533