EGFR (3A9) Mouse mAb

• Catalog Number: orb1563239
• Category: Antibodies
• Description: EGFR (3A9) Mouse mAb
• Target: EGFR
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/ml
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Purification: Affinity Purified
• Immunogen: Synthetic Peptide of EGFR
• UniProt ID: P00533
• MW: Calculated MW: 134 kDa; Observed MW: 170 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
• Antibody Type: Primary Antibody
• Clone Number: 3A9-9D2-9F7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR; ERBB; ERBB1; HER1; Epidermal growth factor r
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of EGFR in Hela using EGFR (3A9) antibody (Left) and DAPI (Right).

Western blot analysis of EGFR (3A9) in Hela lysates using EGFR (3A9) antibody.

Western blot analysis of EGFR (3A9) in Hela, A549 lysates using EGFR (3A9) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using EGFR antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.