EGFR (3A9) Mouse mAb

SKU: orb1563239

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG1
Clone No.	3A9-9D2-9F7
Immunogen	Synthetic Peptide of EGFR
Target	EGFR
Molecular Weight	Calculated MW: 134 kDa; Observed MW: 170 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Quality Guarantee

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Immunofluorescence analysis of EGFR in Hela using EGFR (3A9) antibody (Left) and DAPI (Right).

Western blot analysis of EGFR (3A9) in Hela lysates using EGFR (3A9) antibody.

Western blot analysis of EGFR (3A9) in Hela, A549 lysates using EGFR (3A9) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using EGFR antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.