EGF Receptor / EGFR Antibody

• Catalog Number: orb750123
• Category: Antibodies
• Description: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for this EGFR antibody.
• UniProt ID: P00533
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: ERB59-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green, clone ERB59-1) with DAPI nuclear stain (blue).

FACS testing of A431 cells with isotype control (gray), without primary antibody (black) and EGFR antibody (green, clone ERB59-1).