EGF Receptor / EGFR Antibody

• Catalog Number: orb749489
• Category: Antibodies
• Description: This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human EGF Receptor protein was used as the immunogen for the EGFR antibody.
• UniProt ID: P00533
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Application notes: Optimal dilution of the EGFR antibody should be determined by the researcher.1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: GFR1195
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human placenta stained with EGFR antibody (clone GFR1195).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EGFR antibody (clone GFR1195). These results demonstrate the foremost specificity of the GFR1195 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone GFR1195) as confirmation of integrity and purity.