EEF1A1 Antibody

SKU: orb1263770

Description

EEF1A1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, C. elegans, Drosophila, Gallus, Hamster, Mouse, Rabbit, Rat, Xenopus, Yeast, Zebrafish
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1.
• Target: EEF1A1
• Molecular Weight: 50 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7

Western blot analysis of EEF1A1/ EEF1A2 Antibody in Y79, T47D cell line lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

With 293T cell line lysate, resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 (1:500, 4°C, overnight) and horseradish peroxidase–conjugated second antibo dy (rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).

Quick Database Links

Gene Symbol

EEF1A1

UniProt

P68104

RefSeq:P68104