EEF1A1 Antibody

• Catalog Number: orb1258518
• Category: Antibodies
• Description: EEF1A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EEF1A1
• UniProt ID: P68104
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FL
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (orb1258518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.