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EEF1A1 Antibody
SKU: orb1258518
Description
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1). |
| Target | EEF1A1 |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1
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Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (orb1258518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
EEF1A1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
EEF1A1 Antibody (orb1258518)
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