EEF1A1 Antibody

• Catalog Number: orb1263769
• Category: Antibodies
• Description: EEF1A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Hamster, Mouse, Rabbit, Rat, Xenopus, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: EEF1A1
• UniProt ID: P68104
• NCBI: P68104
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Elongation factor 1-alpha 1, EF-1-alpha-1, Elongat
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EEF1A1 Antibody in MCF-7 cell line lysates (35 ug/lane)

Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of EEF1A1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

EEF1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.