EEF1A1 Antibody

SKU: orb1263769

Description

EEF1A1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus, Hamster, Mouse, Rabbit, Rat, Xenopus, Zebrafish
• Application Notes:
  For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1.
• Target: EEF1A1
• Molecular Weight: 50 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7

Western blot analysis of EEF1A1 Antibody in MCF-7 cell line lysates (35 ug/lane)

Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of EEF1A1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

EEF1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

EEF1A1

UniProt

P68104

RefSeq:P68104