EEF1A1/ EEF1A2 Antibody (N-term)

SKU: orb1929955

Description

EEF1A1/ EEF1A2 Antibody (N-term)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Bovine, C. elegans, Drosophila, Gallus, Hamster, Mouse, Rabbit, Rat, Xenopus, Yeast, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 50141 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

EEF1A, EF1A, LENG7

Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of EEF1A1/ EEF1A2 Antibody (N-term) in Y79, T47D cell line lysates (35 ug/lane). EEF1A1/ EEF1A2 (arrow) was detected using the purified Pab.

With 293T cell line lysate, resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 (abgent, 4°C, overnight) and horseradish peroxidase–conjugated second antibo dy (rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).

Quick Database Links

UniProt

P68104