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Item 1 of 4
Item 1 of 4
EEF1A1/ EEF1A2 Antibody (N-term)
Catalog Number: orb1929955
| Catalog Number | orb1929955 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19607 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | C. elegans, Drosophila, Equine, Hamster, Mouse, Other, Rabbit, Rat, Zebrafish |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 50141 Da |
| Target | This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1. |
| UniProt ID | P68104 |
| NCBI | NP_001393.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Elongation factor 1-alpha 1, EF-1-alpha-1, Elongat Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of EEF1A1/ EEF1A2 Antibody (N-term) in Y79, T47D cell line lysates (35 ug/lane). EEF1A1/ EEF1A2 (arrow) was detected using the purified Pab.
With 293T cell line lysate, resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 (abgent, 4°C, overnight) and horseradish peroxidase–conjugated second antibo dy (rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).
