EBF1 Antibody

• Catalog Number: orb1246460
• Category: Antibodies
• Description: EBF1 Antibody
• Target: EBF1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-HNQLPALANTSV
• UniProt ID: Q9UH73
• Tested applications: ELISA, IP, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml.Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EBF1, early B-cell factor 1 , COE1, EBF, O/E-1, OL
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_076870.1

orb1246460 (0.3 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.