Goat anti-EBF1 / COE1 Antibody

• Catalog Number: orb20040
• Category: Antibodies
• Description: Goat polyclonal antibody to EBF1
• Target: EBF1 / COE1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HNQLPALANTSV
• RRID: AB_10750611
• MW: 64.5
• Tested applications: ELISA, IP, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-0.1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1 μg/ml.Experiment Notes: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti EBF1 antibody, anti early B-cell factor 1 ant
• Note: For research use only
• Entrez: 1879

orb20040 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.