EBAG9 Antibody

• Catalog Number: orb1247575
• Category: Antibodies
• Description: EBAG9 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KEAQRLMKKEQN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 24.4kDa according to NP_004206.1and NP_004206.1). Recommended concentration: 1-3ug/ml. A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EBAG9
• UniProt ID: O00559
• NCBI: NP_004206.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EBAG9, RCAS1, estrogen receptor binding site assoc
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 24.4kDa according to NP_004206.1and NP_004206.1). Recommended concentration: 1-3ug/ml. A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247575 (0.2 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247575 (3.75 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.