EBAG9/RCAS1 Antibody

SKU: orb19280

Description

Goat polyclonal antibody to RCAS1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 26kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 24.4kDa according to NP_004206.1and NP_004206.1). Recommended concentration: 1-3 μg/ml. A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: EBAG9 / RCAS1
• Molecular Weight: 24.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti-BAG9 antibody, anti-Cancer associated surface antigen antibody, anti-Cancer associated surface antigen RCAS1 antibody, anti-Cancer-associated surface antigen RCAS1 antibody, anti-EB9 antibody, anti-EBAG 9 antibody, anti-EBAG9 antibody, anti-Estrogen receptor binding fragment associated gene 9 protein antibody, anti-Estrogen receptor binding site associated antigen 9 antibody, anti-estrogen receptor binding site associated, anti- antigen, anti- 9 antibody, anti-Estrogen receptor-binding fragment-associated gene 9 protein antibody, anti-PDAF antibody, anti-RCAS 1 antibody, anti-RCAS1 antibody, anti-RCAS1_HUMAN antibody, anti-Receptor binding cancer antigen expressed on SiSo cells antibody, anti-Receptor-binding cancer antigen expressed on SiSo cells antibody

Western blot analysis of A431 lysate using RCAS1 antibody

Immunohistochemical staining of Human Skeletal Muscle using RCAS1 antibody

Quick Database Links

Gene Symbol

EBAG9 / RCAS1

NCBI Gene ID

9166