E2F6 Antibody

• Catalog Number: orb1258076
• Category: Antibodies
• Description: E2F6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 38kDa
• Target: E2F6
• UniProt ID: O75461
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: E2F6, E2F-6
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of MCF-7 cells, using E2F6 antibody (orb1258076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human esophageal cancer using E2F6 antibody (orb1258076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using E2F6 antibody (orb1258076) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using E2F6 antibody (orb1258076). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells using 3 ug E2F6 antibody (orb1258076). Western blot was performed from the immunoprecipitate using E2F6 antibody (orb1258076) at a dilution of 1:500.