E2F6 Antibody

SKU: orb1151653

Description

Mouse monoclonal antibody to E2F6

Research Area

Epigenetics

Images & Validation

• Tested Applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the E2F6 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: PCRP-E2F6-1F8
• Immunogen: Recombinant full-length human E2F6 protein was used as the immunogen for the E2F6 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of human HeLa cells using E2F6 antibody (green, clone PCRP-E2F6-1F8) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with E2F6 antibody (blue, clone PCRP-E2F6-1F8), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free E2F6 antibody (PCRP-E2F6-1F8) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using E2F6 antibody (clone PCRP-E2F6-1F8). These results demonstrate the foremost specificity of the PCRP-E2F6-1F8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

O75461