E2F6 Antibody

• Catalog Number: orb213868
• Category: Antibodies
• Description: The E2F6 Antibody is suitable for ChIP, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary.
• UniProt ID: O54917, O75461
• Tested applications: ChIP, IHC, IP, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, ChIP: 1-100-1-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Transcription factor E2F6; E2F-6
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 50496, 1876

Western blot analysis of E2F6 expression in PC12 (A), A2780 (B), HEK293T (C), HCT116 (D) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 35 kD)

Immunohistochemical analysis of E2F6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ChIP analysis of Mouse ES cell nuclear lysate, incubated for 16 hours at 4°C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Tuba3. Negative Control: Beta-Actin. Detection step: Real-time PCR.