cadherin 1 Antibody

SKU: orb1742651

Description

E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.

Images & Validation

• Tested Applications: ICC, IHC, IHC-P, IP, PLA, WB
• Dilution Range: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Reactivity: Canine, Human, Mouse, Rat, Zebrafish
• Application Notes:
  Positive control: MCF-7

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
• Target: cadherin 1
• Molecular Weight: 97
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 0.14 mg/ml (Please refer to the vial label for the specific concentration.)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Quick Database Links

Gene Symbol

cadherin 1

UniProt

P12830