E cadherin Rabbit Polyclonal Antibody

SKU: orb11101

Description

E cadherin Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human E-cadherin (841-882/882aa)
• Target: CDH1
• Molecular Weight: 90/97 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; CADH1_HUMAN.

Histogram of MCF7 cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Sample: Lane 1: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: A549 (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-E cadherin (orb11101) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 120 kD.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-E cadherin (orb11101) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90/97 kD, Observed band size: 125 kD.

Quick Database Links

Gene Symbol

CDH1

UniProt

P12830

References

Gong, Liying et al. Catheter-Based Renal Denervation Attenuates Kidney Interstitial Fibrosis in a Canine Model of High-Fat Diet-Induced Hypertension Kidney Blood Press. Res., (2019)

PubMed: 31291627