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E-cadherin (Intracellular) Antibody
SKU: orb1294381
Description
Images & Validation
−Item 1 of 10
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:1000 Immunofluorescence 1:100-1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Intracellular domain corresponding to Human E-cadherin |
| Target | E-cadherin (Intracellular) |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Anti-E cadherin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294381 overnight at 4°C.
Anti-E cadherin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294381 overnight at 4°C. N-cadherin antibody (orb1294388).
Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: E-cadherin antibody (orb1294381).
Application: Immunofluorescence (ICC/IF), Sample: H1299, Permeabilization: 0.2% Triton X-100, Blocking: 3% BSA blocking for 1 hour, Temperature: RT Fixative: 4% PFA.
Application: Immunohistochemistry (FFPE), Sample: Human Cancer Tissue sectio ns, Antigen retrieval: Heat mediated Tris - Buffer pH9.
Immunofluorescence of paraffin-embedded sections. Cell: mouse colon, DAPI: 1-300, Green: E-cadherin (orb1294381): 1-300 Anti-rabbit 488: 1-300.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294381 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294381 at 1/100.
Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling E-cadherin with orb1294381 at 1/100.
Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling E-cadherin with orb1294381 at 1/100.
Quick Database Links
Gene Symbol
E-cadherin (Intracellular)
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
E-cadherin (Intracellular) Antibody (orb1294381)
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