E-Cadherin Antibody / CDH1

• Catalog Number: orb750019
• Category: Antibodies
• Description: Recognizes a protein of 80-120kDa, identified as E-cadherin/CDH1. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4A2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human protein was used as the immunogen for the E-Cadherin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This E-Cadherin antibody is available for research use only.
• UniProt ID: P12830
• Storage: Store the E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the E-Cadherin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (green, clone 4A2) and Reddot nuclear stain (red).

IHC testing of FFPE human skin with E-Cadherin antibody (clone 4A2). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Western blot testing of human MCF-7 cell lysate with E-Cadherin antibody (clone 4A2). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Flow cytometry testing of human MCF7 cells with E-Cadherin antibody (clone 4A2); Red = isotype control, Blue = E-Cadherin antibody.

SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone 4A2) as confirmation of integrity and purity.