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E-Cadherin Antibody / CDH1

Catalog Number: orb750012

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb750012
CategoryAntibodies
DescriptionRecognizes a protein of 80-120kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
Species/HostMouse
ClonalityMonoclonal
Clone NumberCDH1/1525
Tested applicationsIF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenA full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis E-Cadherin antibody is available for research use only.
UniProt IDP12830
StorageStore the E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Expiration Date12 months from date of receipt.
E-Cadherin Antibody / CDH1

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).

E-Cadherin Antibody / CDH1

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).

E-Cadherin Antibody / CDH1

Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).

E-Cadherin Antibody / CDH1

Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

E-Cadherin Antibody / CDH1

Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (clone CDH1/1525, green) and Reddot nuclear stain (red).

E-Cadherin Antibody / CDH1

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using E-Cadherin antibody (clone CDH1/1525). These results demonstrate the foremost specificity of the CDH1/1525 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

E-Cadherin Antibody / CDH1

SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone CDH1/1525) as confirmation of integrity and purity.

E-Cadherin Antibody / CDH1

Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin antibody (CDH1/1525).

E-Cadherin Antibody / CDH1

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

E-Cadherin Antibody / CDH1

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

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    IF,  IHC-P,  WB

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    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    FC,  IF,  IHC-P,  WB

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    IF,  IHC-P,  WB

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    Mouse

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    FACS,  IF,  IHC-P,  WB

    Human

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    Recombinant

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    20 μg
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    IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg