E-Cadherin Antibody / CDH1

SKU: orb750012

Description

Recognizes a protein of 80-120kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD31 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CDH1/1525
• Immunogen: A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).

Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (clone CDH1/1525, green) and Reddot nuclear stain (red).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using E-Cadherin antibody (clone CDH1/1525). These results demonstrate the foremost specificity of the CDH1/1525 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone CDH1/1525) as confirmation of integrity and purity.

Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Quick Database Links

UniProt

P12830