DUT Antibody

SKU: orb1258016

Description

DUT Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:100 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
• Target: DUT
• Molecular Weight: Observed: 24kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

DUT, FLJ20622, dUTPase

Western blot analysis of extracts of various cell lines, using DUT antibody (orb1258016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (orb1258016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

DUT

UniProt

P33316