dUTPase/DUT Antibody

SKU: orb1248032

Description

dUTPase/DUT Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QLRFARLSEHATAPT
• Target: dUTPase / DUT
• Molecular Weight: Approx 20kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

DUT, deoxyuridine triphosphatase, dUTPase, dUTP nucleotidohydrolase, dUTP pyrophosphatase, deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

orb1248032 (0.1 ug/ml) staining of HEK293 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248032 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248032 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

dUTPase / DUT

UniProt

P33316

RefSeq (Protein):NP_001020419.1, NP_001020420.1, NP_001939.1