DUT Antibody (C-term)

SKU: orb1936036

Description

DUT Antibody (C-term)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 26563 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Western blot analysis of lysate from A431 cell line, using DUT Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Anti-DUT Antibody (C-term) at 1:1000 dilution + SW620 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26. 5 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DUT Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

P33316