DLL1 Antibody

• Catalog Number: orb1246415
• Category: Antibodies
• Description: DLL1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-ATQRHLTVGEEWSQD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). An additional band of nknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration:0.3-7ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DLL1
• UniProt ID: O00548
• NCBI: NP_005609.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delt
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). An additional band of nknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration:0.3-7ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246415 (2 ug/ml) staining of Rat Pancreas lysate (A) + Peptide (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246415 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/membrane staining.

orb1246415 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).