DLL1 Antibody

• Catalog Number: orb20203
• Category: Antibodies
• Description: Goat polyclonal to DLL1
• Target: DLL1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ATQRHLTVGEEWSQD
• RRID: AB_10749263
• MW: 78.1
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-7 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 75kDa band observed in Mouse Heart and Rat Heart lysates (calculated MW of 78.1kDa according to Human NP_005609.3, 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DLL1 antibody, anti delta-like 1 (Drosophila)
• Research Area: Developmental Biology, Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 28514

orb20203 (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of Mouse Heart (A), Rat Pancreas (B) lysate protein using DLL1 antibody

Flow cytometric analysis of HeLa cells using DLL1 antibody