DIO2 Antibody

• Catalog Number: orb1246730
• Category: Antibodies
• Description: DIO2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Predicted Reactivity: Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: EVKKHQNQEDRC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3ug/ml. Data obtained from customer.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DIO2
• UniProt ID: Q92813
• NCBI: NP_001007024.1, NP_054644.1, NP_000784.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DIO2, deiodinase, iodothyronine, type II , 5DII, D
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3ug/ml. Data obtained from customer.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246730 (2 ug/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.

orb1246730 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

orb1246730 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).