DIO2 Antibody (Center)

SKU: orb1928324

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:500-2000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22907
• Immunogen: This DIO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-191 amino acids of human DIO2. Antigen Region: 165-191 aa.
• Target: DIO2
• Molecular Weight: 30552 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase, DIO2, ITDI2, TXDI2

Western blot analysis of DIO2 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane). DIO2 (arrow) was detected using the purified Pab.

DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

DIO2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

All lanes: Anti-DIO2 Antibody (Center) at 1:500-2000 dilution. Lane 1: Mouse heart tissue lysate. Lane 2: Human skeletal muscle tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

DIO2

UniProt

Q92813

RefSeq (Protein):NP_001229432.1, NP_001007024.1, NP_001229431.1, NP_000784.2, NP_054644.1