Goat anti-DIO2 Antibody

• Catalog Number: orb19555
• Category: Antibodies
• Description: Goat polyclonal antibody to DIO2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Reactivity: Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, IHC-P: 2-3 μg/ml
• Conjugation: Unconjugated
• MW: 30.5; 34.6
• Target: DIO2
• Entrez: 1734
• Protein Sequence: EVKKHQNQEDRC
• RRID: AB_10748805
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DIO2 antibody, anti deiodinase, iodothyronine
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3ug/ml. Data obtained from anonymous customer.WB: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5kDa according to NP_NP_000784.2 and of 34.6kDa according to NP_001007024.1. The 65-70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19555 (2ug/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.

Immunofluorescence analysis of HeLa cells using DIO2 antibody

Flow cytometric analysis of MCF7 cells using DIO2 antibody