DDX5 Antibody

SKU: orb1247613

Description

DDX5 Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. This data is from a previous batch, not on sale.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PMIGYPMPTGYSQ
• Target: DDX5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DKFZp434E109, DKFZp686J01190, ATP-dependent RNA helicase DDX5, DEAD box-5

orb1247613 (0.5 ug/ml) staining of cell lines A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and NIH3T3 (E) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247613 (2.5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247613 (2.5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247613 (2.5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

DDX5

UniProt

P17844

RefSeq (Protein):NP_004387.1