DDX5/p68 RNA helicase Antibody

SKU: orb18641

Description

Goat polyclonal antibody to DDX5

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 0.3-1 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1kDa according to NP_004387). Recommended concentration: 0.11-0.3 μg/ml.Experiment Notes: Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DDX5 / p68 RNA helicase
• Molecular Weight: 69.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 0.3-1 μg/ml
• Disclaimer: For research use only

Alternative Names

anti DDX5 antibody, anti P68 antibody, anti HLR1 antibody, anti G17P1 antibody, anti HUMP68 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa) antibody, anti RNA-dependent ATPase antibody, anti DEAD/H box-5 (RNA helicase, 68kD) antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody, anti DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antibody, anti DKFZp434E109 antibody, anti DKFZp686J01190 antibody, anti ATP-dependent RNA helicase DDX5 antibody, anti DEAD box-5 antibody

Western blot analysis of A431 cell lysate using DDX5 antibody

Immunohistochemical staining of Human Prostate using DDX5 antibody

Western blot analysis of NIH3T3 (Lane 1), HeLa (Lane 2), A431 (Lane 3) and Jurkat (Lane 4) using DDX5 antibody

Immunohistochemical staining of Human Uterus using DDX5 antibody

Immunohistochemical staining of Human Breast using DDX5 antibody

Quick Database Links

Gene Symbol

DDX5 / p68 RNA helicase

NCBI Gene ID

1655