DDX5/p68 RNA helicase Antibody

• Catalog Number: orb18641
• Category: Antibodies
• Description: Goat polyclonal antibody to DDX5
• Target: DDX5 / p68 RNA helicase
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Concentration: 0.3-1 μg/ml
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PMIGYPMPTGYSQ
• RRID: AB_10754603
• MW: 69.1
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1kDa according to NP_004387). Recommended concentration: 0.11-0.3 μg/ml.Experiment Notes: Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DDX5 antibody, anti P68 antibody, anti HLR1 a
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 1655
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A431 cell lysate using DDX5 antibody

Immunohistochemical staining of Human Prostate using DDX5 antibody

Western blot analysis of NIH3T3 (Lane 1), HeLa (Lane 2), A431 (Lane 3) and Jurkat (Lane 4) using DDX5 antibody

Immunohistochemical staining of Human Uterus using DDX5 antibody

Immunohistochemical staining of Human Breast using DDX5 antibody