DDX5 Antibody

SKU: orb379628

Description

Mouse monoclonal antibody to DDX5

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Monkey, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: 4F1C12
• Immunogen: Purified recombinant fragment of human DDX5 (AA: 475-614) expressed in E. Coli.
• Target: DDX5
• Molecular Weight: 69.1kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

p68; HLR1; G17P1; HUMP68

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of DDX5 antibody

Flow cytometric analysis of Hela cells using DDX5 antibody

Immunohistochemical staining of human esophageal cancer tissue using DDX5 antibody

Immunohistochemical staining of human bladder cancer tissue using DDX5 antibody

Western blot analysis of HT-29 (Lane 1), Hela (Lane 2), NIH/3T3 (Lane 3), COS7 (Lane 4), SW620 (Lane 5), Jurkat (Lane 6), A431 (Lane 7), and MCF-7 (Lane 8) cell lysate using DDX5 antibody

Western blot analysis of HEK293 cell lysate using DDX5 antibody

Western blot analysis of recombinant protein using DDX5 antibody

Quick Database Links

Gene Symbol

DDX5

UniProt

P17844

NCBI Gene ID

1655