DAG1 Antibody

SKU: orb1249089

Description

DAG1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4kDa according to NP_004384.4). Approx 80kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.9kDa according to NP_034147.1). The observed molecular weights correspond to the glycosylated form of alpha-dystroglycan. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HVGKHEYFMHATDK
• Target: DAG1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1

orb1249089 (0.2 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249089 (0.1 ug/ml) staining of Mouse and Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249089 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Quick Database Links

Gene Symbol

DAG1

UniProt

Q14118

RefSeq (Protein):NP_004384.4