DAG1 Antibody

• Catalog Number: orb1249089
• Category: Antibodies
• Description: DAG1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-HVGKHEYFMHATDK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4kDa according to NP_004384.4). Approx 80kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.9kDa according to NP_034147.1). The observed molecular weights correspond to the glycosylated form of alpha-dystroglycan. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DAG1
• UniProt ID: Q14118
• NCBI: NP_004384.4
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DAG1, dystroglycan 1 (dystrophin-associated glycop
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4kDa according to NP_004384.4). Approx 80kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.9kDa according to NP_034147.1). The observed molecular weights correspond to the glycosylated form of alpha-dystroglycan. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249089 (0.2 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249089 (0.1 ug/ml) staining of Mouse and Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249089 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.