Cytokeratin 10/13 Antibody

• Catalog Number: orb749937
• Category: Antibodies
• Description: This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on tissue sections this mAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 antibody.
• UniProt ID: P13646, P13645
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the CDw17 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: DE-K13
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10/13 antibody (clone DE-K13).

Western blot testing of A431 cell lysate and Cytokeratin 10/13 antibody (clone DE-K13).

SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 10/13 Antibody (clone DE-K13). Confirmation of Integrity and Purity of the Antibody.

Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 10/13 antibody (clone DE-K13, green) and Reddot nuclear stain (red).