Cytokeratin 10/13 Antibody

SKU: orb43753

Description

Mouse Monoclonal to Cytokeratin 10/13.

Images & Validation

• Tested Applications: IHC-Fr, IHC-P, WB
• Reactivity: Feline, Human
• Application Notes:
  Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections): Use at an assay dependent dilution. Western blotting: recommended dilution: 1-3 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: DE-K13
• Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.
• Target: Cytokeratin 10/13
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-Cytokeratin 10+13, (DE-K13). The antibody DE-K13 stains only Cytokeratin 13 when used on formalin-fixed, paraffin-embedded tissue sections.

Quick Database Links

Gene Symbol

Cytokeratin 10/13

NCBI Gene ID

35