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Catalog Number | orb248639 |
---|---|
Category | Antibodies |
Description | This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | C11 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Multi Cytokeratin antibody is available for research use only. |
Entrez | 3860, 3875, 13, 18, 3851, 3852, 6, 3856, 8, 10, 3858, 3853, 4, 5 |
Storage | Store the Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human colon carcinoma using Multi Cytokeratin 4/5/6/8/10/13/18 antibody
FC, IF, IHC, WB | |
Bovine, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Porcine, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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