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Cytokeratin Antibody, Multi 4/5/6/8/10/13/18

Catalog Number: orb248639

DispatchUsually dispatched within 5-10 working days
$ 764.00
Catalog Numberorb248639
CategoryAntibodies
DescriptionThis antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
Species/HostMouse
ClonalityMonoclonal
Clone NumberC11
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenA keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Multi Cytokeratin antibody is available for research use only.
Entrez3860, 3875, 13, 18, 3851, 3852, 6, 3856, 8, 10, 3858, 3853, 4, 5
StorageStore the Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Cytokeratin Antibody, Multi 4/5/6/8/10/13/18

Immunohistochemical staining of Human colon carcinoma using Multi Cytokeratin 4/5/6/8/10/13/18 antibody

  • Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11] [orb1252667]

    FC,  IF,  IHC,  WB

    Bovine, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Porcine, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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