Cyclin B1

• Catalog Number: orb1294340
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin B1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100–1:200
• Conjugation: Unconjugated
• Target: Cyclin B1
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cyclin B1 antibody - at 1/500 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294340 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling Cyclin B1 with orb1294340 at 1/100.

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Cyclin B1 with orb1294340 at 1/100.

Huh-7 cell were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294340 Anti-Cyclin B1 antibody (red) at 1:400 and 4°C.