CCNB1 Antibody

• Catalog Number: orb626160
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin B1
• Target: CCNB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Immunogen: cyclin B1
• UniProt ID: P14635
• MW: 55 kDa
• Tested applications: ELISA, IF, IHC, IP, WB
• Dilution range: WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin
• Note: For research use only

Immunofluorescent analysis of HeLa cells using orb626160 (Cyclin B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-Cyclin B1 (IP: orb626160, 4 ug; Detection: orb626160 1:500) with HeLa cells lysate 1200 ug.

HeLa cells were subjected to SDS PAGE followed by western blot with orb626160 (CCNB1 antibody) at dilution of 1:500.

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using orb626160 (Cyclin B1 Antibody) at dilution of 1:50. heat mediated antigen retrieved with Tris-EDTA buffer (pH9).