CTLA4 Antibody [8A1]

• Catalog Number: orb1239312
• Category: Antibodies
• Description: CTLA4 Antibody [8A1]
• Target: CTLA4
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Purification: CTLA-4 Antibody is supplied as protein A purified IgG1.
• Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
• UniProt ID: P16410
• MW: Predicted: 25 kDa Observed: 32 kDa
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Application notes: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Clone Number: 8A1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte asso
• Note: For research use only
• NCBI: NP_005205

Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1 μg/mL.

Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.

Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [8A1] (orb1239312) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL.

Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [8A1] (orb1239312) Blue: DAPI staining

Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.

Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with orb1239307, orb1239311, and orb1239312 antibodies at decreasing concentrations.