CTLA4 Rabbit Polyclonal Antibody

• Catalog Number: orb156486
• Category: Antibodies
• Description: CTLA4 Rabbit Polyclonal Antibody
• Target: CTLA4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% ProClin300 and 50% Glycerol
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CTLA-4/CD152 (75-170/223aa)
• UniProt ID: P16410
• MW: 21 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD 152; CD152; CD152 antigen; Celiac disease 3; CE
• Research Area: Autoimmune, CD Markers, Cell Biology, Cytotoxic Ce
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC orange), used under the same conditions.

Blank control (blue): Mouse Spleen Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CTLA4 antibody (orb156486), Dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.

Sample: Liver (Mouse) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.

Sample: Liver (Rat) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.