CTLA4 Antibody

SKU: orb1239307

Description

CTLA4 Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 0.125 - 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: [1E6]
• Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
• Target: CTLA4
• Molecular Weight: Predicted: 25 kDa Observed: 32 kDa
• Purification: CTLA-4 Antibody is supplied as protein A purified IgG1.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3

Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.125 and 0.25 μg/mL.

Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.

Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [1E6] (orb1239307) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [1E6] (orb1239307) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [1E6] (orb1239307) Blue: DAPI staining

Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.

Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with orb1239307, orb1239311, and orb1239312 antibodies at decreasing concentrations.

Quick Database Links

Gene Symbol

CTLA4

UniProt

P16410

RefSeq (Protein):NP_005205